在没有溶解之前,厂家生产出来的EDTA二钠大多数为粉末状物质,在使用前需要将其配置成溶液后加入试管中。所以下面具体给大家介绍一下EDTA二钠溶液的配置及注意的一些问题。
Before dissolution, most of the EDTA disodium produced by the manufacturer are powder substances, which need to be prepared into solution before use and then added into the test tube. So let's introduce the configuration of EDTA disodium solution and some problems to be noticed.
一、配置步骤
1、 Configuration steps
将EDTA二钠粉末配置成溶液,其步骤大致如下:
To prepare EDTA disodium powder into solution, the steps are as follows:
1、准备适量的EDTA二钠粉末,蒸馏水、氢氧化钠溶液。
1. Prepare an appropriate amount of EDTA disodium powder, distilled water and sodium hydroxide solution.
2、将准备好的EDTA二钠粉末中加入一定量的蒸馏水,使其溶解,然后用搅拌棒快速搅拌。搅拌过程中加入氢氧化钠溶液,直将PH值调整到需要的范数值,带EDTA二钠溶解后,再次加入蒸馏水,将溶液定容所需要的位置。
2. Add a certain amount of distilled water to the prepared EDTA disodium powder to dissolve it, and then quickly stir it with a stirring rod. During the mixing process, add sodium hydroxide solution until the PH value is adjusted to the required value. After the solution is dissolved with disodium EDTA, add distilled water again, and bring the solution to the required volume.
3、然后用过滤器将溶液一次过滤,即可得到溶液,再将溶液装到容器里,放置于高温下使用,配置好的溶液在4度的温度下可稳定保存1年左右。
3. Then use a filter to filter the solution once to get the solution. Then put the solution into a container and put it under high temperature for sterilization. The prepared solution can be stably stored for about 1 year at 4 ℃.
二、配置注意问题
2、 Configuration considerations
为了不影响EDTA二钠标准溶液的准确度,我们在配置的过程中要注意以下几点:
In order not to affect the accuracy of EDTA disodium standard solution, we should pay attention to the following points in the process of configuration:
1、称取EDTA二钠粉末时,需注意准确控制用量,避免在配置过程中出现误差性。在配置期间所用的溶液必须为蒸馏水配置,不含任何成分杂质,且使用的容器应用纯净水清洗3次以上。
1. When weighing EDTA disodium powder, attention should be paid to accurately control the dosage to avoid errors in the configuration process. The solution used during the preparation must be distilled water, free of any constituent impurities, and the container used should be washed with purified water for more than 3 times.
2、配置完成的溶液不能放在普通玻璃瓶中,因为瓶中的钙离子可能会和EDTA产生反应,会影响实验结果,尽量使用聚乙烯瓶或者硬质玻璃瓶。
2. The prepared solution cannot be placed in ordinary glass bottles because the calcium ions in the bottles may react with EDTA, which will affect the experimental results. Try to use polyethylene bottles or hard glass bottles.
3. The produced EDTA disodium powder is easy to transport and store. If it is prepared into a solution, it should be prepared and used immediately. Do not place the prepared solution for a long time, which will lead to deterioration and abnormal results. Come to our website for more relevant content http://www.jinhao360.com Consult!